![]() Both the forward and reverse sequences obtained were analyzed with BLAST and by manual review. In CRPC, AR-V7 expression is predominantly (94 of cases) nuclear and correlates with AR-FL expression (P 0.001) and AR copy number (P 0.026). Sequencing reaction products were separated electrophoretically on an ABI PRISM 3100 apparatus (Applied Biosystems). PCR products were diluted and cycle sequenced using the BigDye Terminator Cycle Sequencing Kit v. After RT-PCR, free nucleotides and excess primer were removed using PCR Purification kit (QIAGEN). The conditions for RT-PCR were one cycle of 50☌ for 30 minutes, 95☌ for 15 minutes, 45 cycles of 94☌ for 40 seconds, 62☌ for 40 seconds, 72☌ for 1 minute, and one cycle of 72☌ for 10 minutes. ![]() The exon 14 that codes for the juxtamembrane domain of the MET gene was amplified with primer F (5′-TGAAATTGAACAGCGAGCTAAAT-3′) and R (5′- TTGAAATGCACAATCAGGCTAC-3′), in an one-step reverse transcription (RT)-PCR setup with Qiagen OneStep Reverse Transcription-PCR kits (Qiagen) using 4 ng of total RNA. an instrument track Xpand 2 Crack Cymatics Serum Presets Free Reddit 95-53. The cDNA sequence of the MET gene was obtained from GenBank (accession number NM 000245.2). Weve gathered all the free Serum presets we could find online plus all the.
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